Utilising light in assistive reproductive technologies

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Campugan, Carl Adrian (2025) Utilising light in assistive reproductive technologies. PhD thesis, University of Nottingham.

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Abstract

Accurate assessment of oocyte and embryo quality remains a key challenge for in vitro fertilisation (IVF) success, where current methods are often subjective and invasive. This thesis investigates the use of optical tweezers for passive microrheology, revealing stage-specific differences in the embryo microenvironment and viability-related differences in the oocyte microenvironment. It also shows that discrete light wavelengths can alter embryo development in ways undetectable by routine morphology. Collectively, these findings establish optical tweezers as a non-invasive approach to assess developmental potential and emphasise the critical role of regulated light conditions in optimising IVF outcomes.

Item Type: Thesis (University of Nottingham only) (PhD)
Supervisors: Dunning, Kylie
Dholakia, Kishan
Wright, Amanda
Mendonca, Tania
Keywords: Optical tweezers, light, in vitro fertilisation, oocytes, pre-implantation embryo, microrheology
Subjects: R Medicine > RG Gynecology and obstetrics
T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics
Faculties/Schools: UK Campuses > Faculty of Engineering
UK Campuses > Faculty of Engineering > Department of Electrical and Electronic Engineering
Item ID: 81863
Depositing User: Campugan, Carl
Date Deposited: 31 Dec 2025 04:40
Last Modified: 31 Dec 2025 04:40
URI: https://eprints.nottingham.ac.uk/id/eprint/81863

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