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Nawab John, Mohamed S (2024) Molecular evidence of Neo-tetraploids in Arabidopsis thaliana leaf elevated potassium. MRes thesis, University of Nottingham.

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Abstract

Part A:

Crop species that are polyploid are more likely to exhibit unique phenotypes, such as increased organ size and strong resistance to a variety of abiotic stressors that are relevant to the climate. In the model species Arabidopsis thaliana neotetraploids show elevated level in potassium (K+) in leaves. It was noted there is an alteration in well-known components of the K+ homeostasis. Mutational analysis revealed that AKT1, HAK5 and CIPK23 are not responsible for the observed increase. Instead, signalling of abscisic acid might connect with leaf elevated potassium. This review gives a brief outline about whole genome duplication and role of K+ in plants and their distribution. Also, it discusses ABA synthesis, response to neo-tetraploids.

Part B:

Polyploid crop species are prone to display distinctive traits, notably larger organs and robust tolerance to a range of abiotic stresses that are climate-relevant. Neotetraploids of the model species Arabidopsis thaliana have increased potassium (K+) levels in their leaves. Reputable elements of the K+ homeostasis were seen to be altered. It was found through mutational research that the observed increase is not caused by AKT1, HAK5, or CIPK23. Alternatively, abscisic acid signalling may be linked to increased potassium in leaves. Our goal was to determine common and ploidy-specific responses to a K+ uptake transporter deficiency by analyzing molecular alterations in diploid and neo-tetraploid wild type and K-transporter mutants. The eight homozygous mutant seeds(Col-02x, Col-04x, akt1-22x, akt1-24x, hak5-32x, hak5-34x, akt1-2/hak5-32x and akt1-2/hak5-34x)from Arabidopsis thaliana were grown in an artificial medium. Then, the root length were calculated and plotted as a graph by using RSTUDIO (Desktop Version: 2024.04.2+764). The RNA samples were extracted from the roots as well as the shoots. A Nanodrop spectrophotometer was utilized to quantify the amount of RNA present in the shoot. To assess the gene expressions, RNA was converted into complementary DNA and run through a polymerase chain reaction. The gene expressions were examined using agarose gel electrophoresis. In data analytics, the Venn diagram was made to compare several genotypes. PANTHER and ThaleMine were the programs used for gene ontology enrichment analysis and gene annotations. The separated genes serve as instruments that may offer a result for the hypothesis that is being investigated.

Item Type:	Thesis (University of Nottingham only) (MRes)
Supervisors:	Fischer, Sina Harding, Stephen
Keywords:	Arabidopsis thaliana, Neo-tetraploids, potassium, HAK5, AKT1 and K+ elevation
Subjects:	S Agriculture > SB Plant culture
Faculties/Schools:	UK Campuses > Faculty of Science > School of Biosciences
Item ID:	79509
Depositing User:	HARDING, Prof Stephen
Date Deposited:	18 Aug 2025 08:28
Last Modified:	18 Aug 2025 08:28
URI:	https://eprints.nottingham.ac.uk/id/eprint/79509

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