Study of glyceraldehyde-3-phosphate oxidoreductases from archaea and bacteriaTools Montarnal, Amaury (2023) Study of glyceraldehyde-3-phosphate oxidoreductases from archaea and bacteria. PhD thesis, University of Nottingham.
AbstractCarbon monoxide is a greenhouse gas mainly produced by anthropogenic sources, but the electrons liberated for CO oxidation toCO2 have a very low potential of -520 mV. Interestingly, CO can be produced in the syngas by the gasification of renewable sources. Hence, to exploit these low potential electrons and carbon from renewable sources, the general objective of this project was to engineer Escherichia coli to use carbon monoxide as the sole energy and carbon source. One of the engineering points was the heterologous expression in E. coli of the glyceraldehyde-3-phosphate oxidoreductase (GAPOR) to function in the gluconeogenic direction to fix the low potential electrons from the carbon monoxide.
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