Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cellsTools Ekrem, Melis (2020) Development of an optimal xeno-free cGMP growth medium for the culture of corneal stroma-derived mesenchymal stem cells. MRes thesis, University of Nottingham.
AbstractThe characteristic profile of CS-MSCs (Corneal Stroma Derived Mesenchymal Stem Cells) is growing in accordance with their increasing therapeutic interest. In vitro expansion of these cells involves basal media DMEM/F-12 supplemented with 10% FBS (Foetal Bovine Serum) or 10% HPL (Human Platelet Lysate). As animal derived serum can confer a risk of disease transmission, and is subject to batch to batch variability, this does not comply with cGMP (current Good Manufacturing Practice) required animal component – free culture protocols for clinical translation. CS-MSCs were additionally cultured in xeno-free and chemically defined media: Stem X Vivo, StemPro MSC SFM XF, Mesenchymal XF and StemMACS XF to determine whether CS-MSCs were able to proliferate at higher levels than in HPL medium and to aid the development of an optimal xeno-free growth media. CS-MSCs cultured in HPL were further subject to comparison with BM-MSCs (Bone Marrow Derived Mesenchymal Stem Cells) cultured in HPL media to assess MSC characteristics and to highlight which media could be determined as most successful for cell proliferation and morphology.
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