Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detectionTools Corden, Christopher, Shipp, Dustin, Matousek, Pavel and Notingher, Ioan (2018) Fast Raman spectral mapping of highly fluorescing samples by time-gated spectral multiplexed detection. Optics Letters, 43 (23). pp. 5733-5736. ISSN 1539-4794
AbstractWe present a time-gated Raman micro-spectroscopy technique suitable for fast Raman mapping of samples eliciting large laser-induced fluorescence backgrounds. To achieve the required time resolution for effective fluorescence rejection, a picosecond pulsed laser and a single-photon avalanche diode were used. A module consisting of a spectrometer, digital micromirror device, and two prisms was used for high-resolution spectral filtering and multiplexing, which is required for a high chemical specificity and short integration times. With this instrument, we demonstrated time-gated Raman imaging of highly fluorescent samples, achieving acquisition times as short as 3 min for 40×40 pixel resolution images.
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