Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity

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Mahadevan, Vaikom, Campbell, Malcolm, McKeown, Pascal and Bayraktutan, Ulvi (2006) Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity. Cardiovascular Research, 72 . pp. 60-68.

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Abstract

Objective- This study investigated whether differences exist in atherogen-induced migratory behaviors and basal antioxidant enzyme capacity of vascular smooth muscle cells (VSMC)

from human coronary (CA) and internal mammary (IMA) arteries.

Methods- Migration experiments were performed using the Dunn chemotaxis chamber. The prooxidant [NAD(P)H oxidase] and antioxidant [NOS, superoxide dismutase, catalase and

glutathione peroxidase] enzyme activities were determined by specific assays.

Results- Chemotaxis experiments revealed that while both sets of VSMC migrated towards

platelet-derived growth factor-BB (1-50 ng/ml) and angiotensin II (1-50 nM), neither

oxidized-LDL (ox-LDL, 25-100 �g/ml) nor native LDL (100 �g/ml) affected chemotaxis in

IMA VSMC. However, high dose ox-LDL produced significant chemotaxis in CA VSMC

that was inhibited by pravastatin (100 nM), mevastatin (10 nM), losartan (10 nM), enalapril

(1 �M), and MnTBAP (a free radical scavenger, 50��M). Microinjection experiments with

isoprenoids i.e. geranylgeranylpyrophosphate (GGPP) and farnesylpyrophosphate (FPP)

showed distinct involvement of small GTPases in atherogen-induced VSMC migration.

Significant increases in antioxidant enzyme activities and nitrite production along with

marked decreases in NAD(P)H oxidase activity and O2

.- levels were determined in IMA

versus CA VSMC.

Conclusions- Enhanced intrinsic antioxidant capacity may confer on IMA VSMC resistance

to migration against atherogenic agents. Drugs that regulate ox-LDL or angiotensin II levels

also exert antimigratory effects.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/1019276
Keywords: Atherosclerosis, Arteries, Oxygen radicals, Nitric oxide
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Medical Sciences and Graduate Entry Medicine
Depositing User: Sayers, Hazel
Date Deposited: 19 Feb 2007
Last Modified: 04 May 2020 20:30
URI: https://eprints.nottingham.ac.uk/id/eprint/466

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