Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

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Contente, Martina Letizia, Planchestainer, Matteo, Molinari, Francesco and Paradisi, Francesca (2016) Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants. Organic and Biomolecular Chemistry, 14 . pp. 9306-9311. ISSN 1477-0539

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Official URL: http://pubs.rsc.org/en/Content/ArticleLanding/2016/OB/C6OB01629D#!divAbstract

Abstract

A transaminase from Halomonas elongata and four mutants generated by an in silico-based design, were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives resulted excellent substrates, only NO2-acetophenones were transformed into the (S)-amine with high enantioselectivity. The different behaviour of wild-type and mutated transaminases was assessed by in silico substrate binding mode studies.

Item Type:	Article
RIS ID:	https://nottingham-repository.worktribe.com/output/818927
Schools/Departments:	University of Nottingham, UK > Faculty of Science > School of Chemistry
Identification Number:	10.1039/C6OB01629D
Depositing User:	Bramwell, Roseanna
Date Deposited:	29 Nov 2016 10:51
Last Modified:	04 May 2020 18:13
URI:	https://eprints.nottingham.ac.uk/id/eprint/38985

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