Scanning electron microscopy of biomaterials

Tools

McKinlay, K.J., Scotchford, C.A., Grant, D.M., Oliver, J.M., King, John R. and Brown, Paul D. (2004) Scanning electron microscopy of biomaterials. In: Electron Microscopy and Analysis 2003: Proceedings of the Institute of Physics Electron Microscopy and Analysis Group Conference, University of Oxford, 3-5 September 2003. Institute of Physics conference series (179). Institute of Physics Publishing, Bristol, pp. 87-90. ISBN 0750309679

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Abstract

A comparison of conventional high vacuum scanning electron microscopy (HVSEM), environmental SEM (ESEM) and confocal laser scanning microscopy (CLSM) in the assessment of cell-material interactions is made. The processing of cells cultured for conventional HVSEM leads to the loss of morphological features that are retained when using ESEM. The use of ESEM in conjunction with CLSM of the labeled cytoskeleton gives an indication of changes to the cell morphology as a consequence of incubation time and substrate surface features.

Item Type:

Book Section

RIS ID:

https://nottingham-repository.worktribe.com/output/1021674

Keywords:

SEM ESEM CLSM Biomaterials

Schools/Departments:

University of Nottingham, UK > Faculty of Engineering > Department of Mechanical, Materials and Manufacturing Engineering

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