Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity

Mahadevan, Vaikom and Campbell, Malcolm and McKeown, Pascal and Bayraktutan, Ulvi (2006) Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity. Cardiovascular Research, 72 . pp. 60-68.

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Objective- This study investigated whether differences exist in atherogen-induced migratory behaviors and basal antioxidant enzyme capacity of vascular smooth muscle cells (VSMC) from human coronary (CA) and internal mammary (IMA) arteries. Methods- Migration experiments were performed using the Dunn chemotaxis chamber. The prooxidant [NAD(P)H oxidase] and antioxidant [NOS, superoxide dismutase, catalase and glutathione peroxidase] enzyme activities were determined by specific assays. Results- Chemotaxis experiments revealed that while both sets of VSMC migrated towards platelet-derived growth factor-BB (1-50 ng/ml) and angiotensin II (1-50 nM), neither oxidized-LDL (ox-LDL, 25-100 g/ml) nor native LDL (100 g/ml) affected chemotaxis in IMA VSMC. However, high dose ox-LDL produced significant chemotaxis in CA VSMC that was inhibited by pravastatin (100 nM), mevastatin (10 nM), losartan (10 nM), enalapril (1 M), and MnTBAP (a free radical scavenger, 50M). Microinjection experiments with isoprenoids i.e. geranylgeranylpyrophosphate (GGPP) and farnesylpyrophosphate (FPP) showed distinct involvement of small GTPases in atherogen-induced VSMC migration. Significant increases in antioxidant enzyme activities and nitrite production along with marked decreases in NAD(P)H oxidase activity and O2 .- levels were determined in IMA versus CA VSMC. Conclusions- Enhanced intrinsic antioxidant capacity may confer on IMA VSMC resistance to migration against atherogenic agents. Drugs that regulate ox-LDL or angiotensin II levels also exert antimigratory effects.

Item Type:Article
Uncontrolled Keywords:Atherosclerosis, Arteries, Oxygen radicals, Nitric oxide
Schools/Departments:University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Medicine > Division of Medical Sciences and Graduate Entry Medicine
ID Code:466
Deposited By:Sayers, Hazel
Deposited On:19 Feb 2007
Last Modified:15 Aug 2013 08:35

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