Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

Contente, Martina Letizia and Planchestainer, Matteo and Molinari, Francesco and Paradisi, Francesca (2016) Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants. Organic and Biomolecular Chemistry, 14 . pp. 9306-9311. ISSN 1477-0539

[img] PDF - Repository staff only until 6 September 2017. - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (639kB)

Abstract

A transaminase from Halomonas elongata and four mutants generated by an in silico-based design, were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives resulted excellent substrates, only NO2-acetophenones were transformed into the (S)-amine with high enantioselectivity. The different behaviour of wild-type and mutated transaminases was assessed by in silico substrate binding mode studies.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Chemistry
Identification Number: https://doi.org/10.1039/C6OB01629D
Depositing User: Bramwell, Roseanna
Date Deposited: 29 Nov 2016 10:51
Last Modified: 01 Dec 2016 05:05
URI: http://eprints.nottingham.ac.uk/id/eprint/38985

Actions (Archive Staff Only)

Edit View Edit View