Hydrodynamic and Microscopic Investigations of Polysaccharides and Glycopeptide Antibiotics of Ophthalmological ImportanceTools Chun, Taewoo (2023) Hydrodynamic and Microscopic Investigations of Polysaccharides and Glycopeptide Antibiotics of Ophthalmological Importance. PhD thesis, University of Nottingham.
AbstractThe thesis consists of two main themes: the eye-drop study for dry eye syndrome and the antibiotic study for antibiotic resistance. Hyaluronic acid (HA) has been commonly used in eyedrop formulations. A difficulty has been its short residence time on ocular surfaces due to ocular clearance mechanisms which remove the polysaccharide almost immediately. To prolong its retention time, tamarind seed gum polysaccharide (TSP) is mixed as a helper biopolymer with HA. Here I look at the hydrodynamic characteristics of HA and TSP (weight average molar mass Mw and viscosity [η]) and then explore the compatibility of these polymers, including the possibility of potentially harmful aggregation effects. The research is based on a novel combination of three methods: sedimentation velocity in the analytical ultracentrifuge (SV-AUC), size-exclusion chromatography coupled to multiangle light scattering (SEC-MALS) and capillary viscometry. For the mixed HA-TSP systems, SEC-MALS indicates the possibility of a weak interaction. However, further investigation using sedimentation coefficient (s) distributions obtained from SV-AUC measurements together with intrinsic viscosity demonstrated no evidence of any significant aggregation phenomenon, reassuring in terms of eye-drop formulation technology involving these substances.
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