Molecular biology of human DDX49 - novel biochemical activities

Anandavijayan, Sabesan (2023) Molecular biology of human DDX49 - novel biochemical activities. MRes thesis, University of Nottingham.

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DExD-box helicases belong to superfamily II of RNA helicases and are characterised by a conserved Asp-Glu-X-Asp motif in their primary protein sequence. These helicases are often involved in pre-mRNA splicing, ribosome biogenesis and also cell cycle mediation; as such, they are implicated in the oncogenesis and proliferation of various cancer types. The overexpression of DEAD-box protein 49 (DDX49) has been associated with a favourable prognosis in cervical cancer patients, however, its structure and function has yet to be fully elucidated. We report that DDX49 is a dual function helicase and nuclease, and discuss how CRISPR-Cas9 mediated downregulation of DDX49 may affect the proliferation and viability of U2OS osteosarcoma cells.

Item Type: Thesis (University of Nottingham only) (MRes)
Supervisors: Bolt, Edward
Killelea, Tom
Keywords: DEAD-box protein 49; Cell proliferation; Dual function helicase and nuclease
Subjects: Q Science > QP Physiology > QP1 Physiology (General) including influence of the environment
Faculties/Schools: UK Campuses > Faculty of Medicine and Health Sciences > School of Life Sciences
Item ID: 72946
Depositing User: Anandavijayan, Sabesan
Date Deposited: 31 Jul 2023 04:40
Last Modified: 31 Jul 2023 04:40

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