Carbon Nanotubes for the Rapid Detection of Intracellular Hydrogen Peroxide and its Application in Bacterial DetectionTools Hicks, Jacqueline (2019) Carbon Nanotubes for the Rapid Detection of Intracellular Hydrogen Peroxide and its Application in Bacterial Detection. PhD thesis, University of Nottingham.
AbstractThe aim of this work is to develop a biosensor capable of detecting bacteria through the specific detection of intracellular hydrogen peroxide. When bacteria interact with host phagocytic immune cells there is a burst of reactive oxygen species known as the respiratory burst. Traditionally this is thought to be a long and extended production of reactive oxygen species. Recent advances however, have shown that the respiratory burst can be more dynamic with a fast and rapid response to bacteria. Conventional techniques for the measurement of reactive oxygen species are limitedin their ability to differentiate between reactive oxygen species and to detect them in a time sensitive manner. In order to fulfil this need for a rapid sensor, an electrochemical nano-biosensor was developed that can interface with cells interacting with the cell cytoplasm and detect hydrogen peroxide in a time and concentration dependent manner. Such a development is a vital first step in the production of a rapid diagnostic device that will aid physicians in correctly treating bacterial infection and will be of particular use in the treatment of sepsis.
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