N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis

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Fray, Rupert G (2018) N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis. Cell Reports, 25 . pp. 1-12. ISSN 2211-1247 (In Press)

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Official URL: https://www.cell.com/cell-reports/home

Abstract

N6-methyladenosine (m6A) is a dynamic, reversible,

covalently modified ribonucleotide that occurs predominantly

toward 30 ends of eukaryotic mRNAs

and is essential for their proper function and regulation.

In Arabidopsis thaliana, many RNAs contain at

least one m6A site, yet the transcriptome-wide function

of m6A remains mostly unknown. Here, we show

that manym6A-modified mRNAs in Arabidopsis have

reduced abundance in the absence of this mark. The

decrease in abundance is due to transcript destabilization

caused by cleavage occurring 4 or 5 nt directly

upstream of unmodified m6A sites. Importantly, we

also find that, upon agriculturally relevant salt treatment,

m6A is dynamically deposited on and stabilizes

transcripts encoding proteins required for salt

and osmotic stress response. Overall, our findings

reveal that m6A generally acts as a stabilizing mark

through inhibition of site-specific cleavage in plant

transcriptomes, and this mechanism is required

for proper regulation of the salt-stress-responsive

transcriptome.

Item Type:

Article

Schools/Departments:

University of Nottingham, UK > Faculty of Science > School of Biosciences
University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences

Identification Number:

https://doi.org/10.1016/j.celrep.2018.10.020

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