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Fray, Rupert G (2018) N6-Methyladenosine Inhibits Local Ribonucleolytic Cleavage to Stabilize mRNAs in Arabidopsis. Cell Reports, 25 . pp. 1-12. ISSN 2211-1247 (In Press)

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Abstract

N6-methyladenosine (m6A) is a dynamic, reversible,

covalently modified ribonucleotide that occurs predominantly

toward 30 ends of eukaryotic mRNAs

and is essential for their proper function and regulation.

In Arabidopsis thaliana, many RNAs contain at

least one m6A site, yet the transcriptome-wide function

of m6A remains mostly unknown. Here, we show

that manym6A-modified mRNAs in Arabidopsis have

reduced abundance in the absence of this mark. The

decrease in abundance is due to transcript destabilization

caused by cleavage occurring 4 or 5 nt directly

upstream of unmodified m6A sites. Importantly, we

also find that, upon agriculturally relevant salt treatment,

m6A is dynamically deposited on and stabilizes

transcripts encoding proteins required for salt

and osmotic stress response. Overall, our findings

reveal that m6A generally acts as a stabilizing mark

through inhibition of site-specific cleavage in plant

transcriptomes, and this mechanism is required

for proper regulation of the salt-stress-responsive

transcriptome.

Item Type:	Article
Schools/Departments:	University of Nottingham, UK > Faculty of Science > School of Biosciences University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences
Identification Number:	10.1016/j.celrep.2018.10.020
Related URLs:	URL URL Type https://doi.org/10.1016/j.celrep.2018.10.020 UNSPECIFIED
Depositing User:	Fray, Rupert
Date Deposited:	31 Oct 2018 15:26
Last Modified:	05 Feb 2019 04:30
URI:	https://eprints.nottingham.ac.uk/id/eprint/55405

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