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Low, Sze Shin (2018) Graphene/zinc oxide nanocomposite : a versatile platform for electrochemical-based genosensor. PhD thesis, University of Nottingham.

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Abstract

In this work, a versatile electrochemical biosensing platform was developed based on graphene/zinc oxide (G/ZnO) nanocomposite. For the synthesis of G/ZnO nanocomposite, two facile and green approaches were employed to eradicate the issues associated with conventional methods, which use harsh chemicals and high temperature. The G/ZnO nanocomposite synthesised via low temperature hydrothermal growth method exhibited approximate 58 times improvement in terms of sensitivity as compared to the G/ZnO nanocomposite synthesised via the mechanical stirring method. Therefore, the G/ZnO nanocomposite with higher sensitivity was employed for the following work.

Results from cyclic voltammetry and amperometry showed that the G/ZnO-modified electrode displayed a wide linear range up to 15 mM for the detection of H2O2 and sensitivity improvements of 200% in comparison to the control sample. Subsequently, an electrochemical genosensor based on G/ZnO nanocomposite was fabricated for the detection of Avian Influenza H5N1 virus. The G/ZnO-based genosensor displayed its potential in replacing the conventional detection method, with result showing higher sensitivity and efficiency. The G/ZnO-based genosensor was further applied for the detection of Coconut Cadang-Cadang Viroid disease (CCCVd) single stranded RNA (ssRNA). Promising results were obtained with high specificity in discriminating the target from mismatched ssRNA sequences.

Item Type:	Thesis (University of Nottingham only) (PhD)
Supervisors:	Tan, Michelle Khiew, Poi Sim Loh, Hwei San
Keywords:	biosensors, nanocomposites, graphene, nanotubes
Subjects:	R Medicine > R Medicine (General) > R855 Medical technology. Biomedical engineering. Electronics T Technology > TK Electrical engineering. Electronics Nuclear engineering T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics
Faculties/Schools:	University of Nottingham, Malaysia > Faculty of Science and Engineering — Engineering > Department of Electrical and Electronic Engineering
Item ID:	46795
Depositing User:	LOW, SZE SHIN
Date Deposited:	05 Sep 2018 11:08
Last Modified:	06 May 2020 10:49
URI:	https://eprints.nottingham.ac.uk/id/eprint/46795

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