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Mistry, Hiten D., Bramham, Kate, Weston, Andrew, Ward, Malcolm, Thompson, Andrew and Chappell, Lucy C. (2013) Urine protein concentration estimation for biomarker discovery. Pregnancy Hypertension, 3 (4). pp. 211-214. ISSN 2210-7797

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Abstract

Recent advances have been made in the study of urinary proteomics as a diagnostic tool for renal disease and pre-eclampsia which requires accurate measurement of urinary protein. We compared different protein assays (Bicinchoninic acid (BCA), Lowry and Bradford) against the ‘gold standard’ amino-acid assay in urine from 43 women (8 non-pregnant, 34 pregnant, including 8 with pre-eclampsia. BCA assay was superior to both Lowry and Bradford assays (Bland Altman bias: 0.08) compared to amino-acid assay, which performed particularly poorly at higher protein concentrations. These data highlight the need to use amino-acid or BCA assays for unprocessed urine protein estimation.

Item Type:	Article
RIS ID:	https://nottingham-repository.worktribe.com/output/1001113
Keywords:	Protein concentration assays; Proteomics; Urine
Schools/Departments:	University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Child Health, Obstetrics and Gynaecology
Identification Number:	https://doi.org/10.1016/j.preghy.2013.07.001
Depositing User:	Mistry, Hiten
Date Deposited:	21 Jul 2017 09:47
Last Modified:	04 May 2020 20:18
URI:	https://eprints.nottingham.ac.uk/id/eprint/44323

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