Cell allocation patterns during mammalian pre-implantation development

Sepulveda-Rincon, Lessly P. (2017) Cell allocation patterns during mammalian pre-implantation development. PhD thesis, University of Nottingham.

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The first cell differentiation in the mammalian embryo occurs during pre implantation development. At blastocyst stage two cell lineages can be distinguished: the inner cell mass (ICM), situated at the embryonic pole and the trophectoderm (TE) at the abembryonic pole. In murine embryos, it has been suggested that the first cleavage plane might be related with the embryonic-abembryonic (Em-Ab) axis at the blastocyst stage. So the daughter cells of the two-cell embryo might be already predisposed to a specific cell lineage further on development. The objective of the present thesis is to investigate the effects of assisted reproductive technologies (ART) factors affecting cell allocation patterns during pre implantation development.

This was addressed by observing cell allocation patterns during mammalian pre implantation development on embryos produced using different ARTs. Using live-cell tracing, it has been concluded that cell allocation patterns during pre-implantation embryo development are potentially conserved among mammals, or at least among mouse and bovine embryos. Pre determined (orthogonal and deviant patterns), as well as stochastic development (random pattern), have been identified in mouse and bovine embryos. The incidence of these cell allocation patterns was not affected by maternal age, oocyte production, oocyte fertilisation/activation method, cleavage-stage biopsy or species.

In addition, differences on epigenetic profiles, coping mechanisms after cell removal and further organ development were present among patterned embryos. Future work is advised to understand the basis of the mechanism(s) driving or driven by cell allocation patterns; particularly its relation with organ development.

Item Type: Thesis (University of Nottingham only) (PhD)
Supervisors: Maalouf, W.
Campbell, B.
Beaujean, Nathalie
Keywords: Mammalian embryo, Pre-implantation development, Cell allocation patterns, Embryo biopsy, Offspring
Subjects: Q Science > QL Zoology > QL951 Embryology
Faculties/Schools: UK Campuses > Faculty of Medicine and Health Sciences > School of Medicine
Item ID: 43031
Depositing User: Sepulveda Rincon, Lessly
Date Deposited: 10 Oct 2017 11:55
Last Modified: 12 Oct 2017 22:49
URI: https://eprints.nottingham.ac.uk/id/eprint/43031

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