Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology

Taresco, Vincenzo, Louzao, Iria, Scurr, David J., Turpin, Eleanor R., Laughton, Charles A., Alexander, Cameron, Burley, Jonathan C. and Garnett, Martin (2017) Rapid nano-gram scale screening method of micro-arrays to evaluate drug-polymer blends using high-throughput printing technology. Molecular Pharmaceutics, 14 (6). pp. 2079-2087. ISSN 1543-8384

Full text not available from this repository.


A miniaturized, high-throughput assay was optimized to screen polymer-drug solid dispersions using a 2-D Ink-jet printer. By simply printing nanoliter amounts of polymer and drug solutions onto an inert surface, drug:polymer micro-dots of tunable composition were produced in an easily-addressable micro-array format. The amount of material printed for each dried spot ranged from 25 ng to 650 ng. These arrays were used to assess the stability of drug:polymer dispersions with respect to recrystallization, using polarized light microscopy. One array with a panel of 6 drugs formulated at different ratios with Poly (vinylpyrrolidone-vinyl acetate) copolymer (PVPVA) was developed to estimate a possible bulk (gram-scale) approximation threshold from the final printed nano amount of formulation. Another array was printed at a fixed final amount of material to establish a literature comparison of one drug formulated with different commercial polymers for validation. This new approach may offer significant efficiency in pharmaceutical formulation screening, with each experiment in the nano-micro-array format requiring from 3 up to 6 orders of magnitude lower amounts of sample than conventional screening methods.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/860398
Additional Information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Medicinal Chemistry Letters copyright © 2017 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.7b00182
Keywords: High throughput array, Ink-jet printing, Amorphous dispersion, Recrystallisation, Polarizing microscopy
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: https://doi.org/10.1021/acs.molpharmaceut.7b00182
Depositing User: Garnett, Martin
Date Deposited: 17 May 2017 13:51
Last Modified: 04 May 2020 18:45
URI: https://eprints.nottingham.ac.uk/id/eprint/42908

Actions (Archive Staff Only)

Edit View Edit View