From sequence to function: insights from natural variation in budding yeasts

Nieduszynski, Conrad A. and Liti, Gianni (2011) From sequence to function: insights from natural variation in budding yeasts. BBA - Biochimica et Biophysica Acta, 1810 (10). pp. 959-966. ISSN 0006-3002

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Natural variation offers a powerful approach for assigning function to DNA sequence—a pressing challenge in the age of high throughput sequencing technologies.

Scope of Review

Here we review comparative genomic approaches that are bridging the sequence–function and genotype–phenotype gaps. Reverse genomic approaches aim to analyse sequence to assign function, whereas forward genomic approaches start from a phenotype and aim to identify the underlying genotype responsible.

Major Conclusions

Comparative genomic approaches, pioneered in budding yeasts, have resulted in dramatic improvements in our understanding of the function of both genes and regulatory sequences. Analogous studies in other systems, including humans, demonstrate the ubiquity of comparative genomic approaches. Recently, forward genomic approaches, exploiting natural variation within yeast populations, have started to offer powerful insights into how genotype influences phenotype and even the ability to predict phenotypes.

General Significance

Comparative genomic experiments are defining the fundamental rules that govern complex traits in natural populations from yeast to humans.

Item Type: Article
Keywords: Saccharomyces cerevisiae; Forward genomics; Reverse genomics; Functional analysis; Quantitative trait locus; Comparative genomics
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences > School of Biology
Identification Number:
Depositing User: Liu, Mr Zhenxing
Date Deposited: 31 Mar 2014 10:51
Last Modified: 04 May 2020 20:23

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