Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthanTools Almutairi, Fahad M., Erten, Tayyibe, Adams, Gary G., Hayes, Maria, McLoughlin, Pádraig, Kök, M. Şamil, Mackie, Alan R., Rowe, Arthur J. and Harding, Stephen E. (2015) Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan. Carbohydrate Polymers, 122 . pp. 359-366. ISSN 0144-8617 Full text not available from this repository.AbstractChitosan, a soluble polycationic derivative of insoluble chitin, has been widely considered for use in the food, cosmetic and pharmaceutical industries. Commercial (“C”) and in-house laboratory (“L”) prepared chitosan samples extracted from crustaceous shells with different molecular weight and degrees of acetylation (25% and 15%) were compared with regards to (i) weight–average molecular weight (Mw); (ii) sedimentation coefficient (so20,w) distribution, and (iii) intrinsic viscosity ([η]). These parameters were estimated using a combination of analytical ultracentrifugation (AUC), size exclusion chromatography coupled to multi-angle laser light scattering (SEC–MALS) and differential pressure viscometry. Polydisperse distributions were seen from sedimentation coefficient distributions and elution profiles from SEC–MALS. Mw values obtained for each sample by sedimentation equilibrium measurements were in excellent agreement with those obtained from SEC–MALS. Mark–Houwink–Kuhn–Sakurada (MHKS) and Wales van Holde analyses of the data all suggest a semi-flexible conformation.
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