Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle

Leigh, James A., Egan, Sharon A., Ward, Philip N., Field, Terence R. and Coffey, Tracey J. (2010) Sortase anchored proteins of Streptococcus uberis play major roles in the pathogenesis of bovine mastitis in dairy cattle. Veterinary Research, 41 (5). 63/1-63/16. ISSN 1297-9716

Full text not available from this repository.

Abstract

Streptococcus uberis, strain 0140J, contains a single copy sortase A (srtA), encoding a transamidase capable of covalently anchoring specific proteins to peptidoglycan. Unlike the wild-type, an isogenic mutant carrying an inactivating ISS1 insertion within srtA was only able to infect the bovine mammary gland in a transient fashion. For the first 24 h post challenge, the srtA mutant colonised at a similar rate and number to the wild type strain, but unlike the wild type did not subsequently colonise in higher numbers. Similar levels of host cell infiltration were detected in response to infection with both strains, but only in those mammary quarters infected with the wild type strain were clinical signs of disease evident. Mutants that failed to express individual sortase substrate proteins (sub0135, sub0145, sub0207, sub0241, sub0826, sub0888, sub1095, sub1154, sub1370, and sub1730) were isolated and their virulence determined in the same challenge model. This revealed that mutants lacking sub0145, sub1095 and sub1154 were attenuated in cattle. These data demonstrate that a number of sortase anchored proteins each play a distinct, non-redundant and important role in pathogenesis of S. uberis infection within the lactating bovine mammary gland.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/706477
Keywords: mastitis, S. uberis, virulence, sortase, vaccine
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: https://doi.org/10.1051/vetres/2010036
Depositing User: Egan, Sharon
Date Deposited: 18 Oct 2016 09:50
Last Modified: 04 May 2020 16:29
URI: https://eprints.nottingham.ac.uk/id/eprint/37635

Actions (Archive Staff Only)

Edit View Edit View