Microcarriers with Complex Architectures Manufactured by Two-Photon Lithography for Mechanobiological Manipulation and Expansion of Mesenchymal Stem CellsTools Hutchinson, Jason (2023) Microcarriers with Complex Architectures Manufactured by Two-Photon Lithography for Mechanobiological Manipulation and Expansion of Mesenchymal Stem Cells. PhD thesis, University of Nottingham.
AbstractMesenchymal stem cells (MSCs) are a powerful tool in regenerative medicine owing to their innate capacity to differentiate into a range of cell lineages and this behaviour has been utilised as a means of tissue repair and regeneration. The prevalent issue in many treatments is the vast number of cells required for therapeutic effect, but this can be addressed through expansion of cell populations in vitro to suitable levels. Microcarriers are designed to provide a high level of cell growth surface within a small volume and have become one of the most promising expansion tools to date. However, transition to approaches that integrate biomechanical cues to modulate cell responses can lead to far greater outcomes than those that can be achieved through surface area alone. Such biophysical properties that can be integrated include geometry, roughness, topography, stiffness, and porosity which can promote specific biological responses through mechanotransduction pathways. This thesis focuses on employing this approach to microcarrier technology and examining the effects of such structures on cell control and enhancement of expansion yield to facilitate MSC production for therapeutic uses.
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