Investigating how tenascin-C regulates miR-155 expression

Zordan, N (2021) Investigating how tenascin-C regulates miR-155 expression. PhD thesis, University of Nottingham.

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Abstract

Innate immunity is deeply reliant on post-transcriptional gene expression modulators such as microRNAs (miRNAs). miRNAs, such as miR-155, are involved in all levels of immune cell development, function and response, causing excessive inflammation and associating with several diseases when dysregulated.

Tenascin-C is an extracellular matrix (ECM) glycoprotein specifically induced in lipopolysaccharide (LPS)-activated macrophages. It was found that tenascin-C was able to modulate miR-155 expression in bone marrow-derived macrophages (BMDMs) from mouse, consequently regulating pro-inflammatory cytokines, such as TNF-α. However, studies focusing on miRNA expression regulated by the ECM are still in their infancy, and how tenascin-C could regulate miR-155 expression remained unknown.

This work aimed to study the mechanism and form of tenascin-C involved in promoting miR-155 expression, specifically at what stage of miR-155 biogenesis tenascin-C was required and if the ECM-associated tenascin-C was the main actor in miR-155 regulation. Moreover, we aimed to identify the membrane receptor associating with tenascin-C during this regulation.

We demonstrated that tenascin-C was involved in regulating miR-155 specifically by activating receptors located on the plasma membrane of tenascin-C knockout (tnc-/-) and wild type (tnc+/+) macrophages from mice, induced with different pathogenic and sterile inflammation stimuli and measured by qPCR and northern blotting. Furthermore, lack of tenascin-C affected miR-155 expression post-transcriptionally, reducing precursor miRNA (pre-miR-155) availability in the cytoplasm, possibly influencing deficient Drosha-mediated cleavage of the primary miRNA (pri-miR-155), as determined by qPCR, northern blot and RNA immunoprecipitation.

Additionally, using a murine 3D cell culture model of ECM and inflammation developed by us, we could identify insoluble ECM-assembled tenascin-C as the form required for LPS-induced miR-155 regulation in macrophages. Indeed, ECM-assembled but not soluble tenascin-C was able to rescue miR-155 expression in tnc-/- BMDMs.

Finally, we identified a possible novel candidate cell-surface co-receptor or antagonist for tenascin-C that, when silenced enhanced miR-155 expression.

Altogether, this work reveals a novel regulatory mechanism of the inflammatory response to infection and identify a potential therapeutic target that could unveil new ways to control excessive inflammation.

Item Type: Thesis (University of Nottingham only) (PhD)
Supervisors: Piccinini, AM
Heery, DM
Keywords: microRNAs, tenascin-C, glycoproteins, immune system
Subjects: Q Science > QP Physiology > QP501 Animal biochemistry
Q Science > QR Microbiology > QR180 Immunology
Faculties/Schools: UK Campuses > Faculty of Science > School of Pharmacy
Item ID: 65645
Depositing User: Zordan, Nicole
Date Deposited: 04 Aug 2021 04:42
Last Modified: 04 Aug 2021 04:42
URI: http://eprints.nottingham.ac.uk/id/eprint/65645

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