Impact of polyphenols on lipid accumulation within skeletal muscle cells

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Alhodieb, Fahad (2020) Impact of polyphenols on lipid accumulation within skeletal muscle cells. PhD thesis, University of Nottingham.

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Abstract

Obesity is characterised by a high-fat accumulation in many organs in the body and is associated with many diseases, including Type 2 diabetes mellitus (T2DM). As most T2DM patients, the major class of diabetes, are obese, researchers have started investigating the role of obesity in this disease. The skeletal muscles are the major organ in the disposal of blood sugar. Also, the accumulation of fats in non-adipocyte cells such as skeletal muscles can result in insulin resistance. The overall aims of this thesis were to establish a useful in vitro model to investigate intracellular lipids accumulation and the effects on insulin sensitivity. Besides, to examine whether dietary polyphenols have a beneficial effect on lipid accumulation and, subsequently, leads to amelioration in insulin sensitivity. C2C12 and L6 myotubes cells were exposed to 0.2mM of different fatty acids; palmitic acid (PA), oleic acid (OA), linoleic acid (LA), and a combination of these FAs (3xFA) total 0.2mM for 3 days, followed by Nile-Red staining. There was a consistently higher deposition of lipids in the LA treated cells after 3 days for both cell lines (P>0.001 and P<0.0001, respectively). C2C12 cells were treated with 0.2mM LA, which resulted in no significant increase in the mRNA of the peroxisome proliferator-activated receptor gamma-2 (PPAR2) (P>0.05) and fatty acid-binding protein 4 (FABP4) (not detectable), which suggests that no adipocytes were occurring. L6 myotubes were treated with 0.2mM LA combined with different polyphenols (0.05µM, 0.1µM, and 1µM of resveratrol, quercetin, or epigallocatechin gallate (EGCG)) for 3 days. Only 1µM EGCG significantly inhibited the lipid deposition (P<0.01). Interestingly, LA treatment (0.2mM) of L6 myotubes for 3 days significantly decreased the insulin-stimulated glucose uptake (P<0.05), while this effect of LA was blocked after co-treatment with 1µM EGCG.

Item Type: Thesis (University of Nottingham only) (PhD)
Supervisors: Parr, Tim
Brameld, John
Keywords: lipid metabolism, polyphenols, lipids--metabolism, plant polyphenols, dietary polyphenols
Subjects: Q Science > QP Physiology
Faculties/Schools: UK Campuses > Faculty of Science > School of Biosciences
Item ID: 61224
Depositing User: ALHODIEB, FAHAD
Date Deposited: 29 Sep 2023 06:47
Last Modified: 29 Sep 2023 06:47
URI: https://eprints.nottingham.ac.uk/id/eprint/61224

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