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Mohd Amin, Nur Amalina (2019) Towards Polyionic Liquid-Based Supports for Application in Biocatalysis. PhD thesis, University of Nottingham.

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Abstract

This thesis describes the utilisation of PILs as the carrier matrices to improve the stability of transaminase enzymes in catalysing chiral amine formation and to ease their subsequent removal from reaction media. The synthesis of the ionic liquid monomers having polymerising groups on the cations has been investigated. These ionic liquid monomers act as the main components for immobilised enzyme carrier matrices. This has included ionic liquids which consisted of variable alkyl chain length on the cation and ionic liquids with different cation and anion combinations. The effects of ionic liquid anions and cations variations to their structure, thermal and optical properties were assessed by using the instrumentation techniques.

The transaminases have been immobilised into PIL matrices by encapsulation method. The influence of various type of PILs to the difference of morphological structure, elemental and optical properties were determined. The suitability of the immobilised transaminases towards the production of chiral amines were examined. This involved the screening process to evaluate the suitability of the PIL support with the enzymes. The effectiveness of the immobilised transaminases to the free transaminases in catalysing the chiral amine formations was investigated. Several parameters of enzymatic reactions were reported including the pH, temperature and reusability studies.

Item Type:	Thesis (University of Nottingham only) (PhD)
Supervisors:	Licence, Peter O'Reilly, Elaine
Keywords:	Polyionic Liquid-Based Supports, PILs, biocatalysis, enzymes
Subjects:	Q Science > QD Chemistry > QD241 Organic chemistry Q Science > QP Physiology > QP501 Animal biochemistry
Faculties/Schools:	UK Campuses > Faculty of Science > School of Chemistry
Item ID:	56798
Depositing User:	Mohd Amin, Nur
Date Deposited:	04 Oct 2023 10:18
Last Modified:	04 Oct 2023 10:18
URI:	https://eprints.nottingham.ac.uk/id/eprint/56798

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