Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells

Sinjab, Faris and Awuah, Dennis and Gibson, Graham and Padgett, Miles and Ghaemmaghami, Amir M. and Notingher, Ioan (2018) Holographic optical trapping Raman micro-spectroscopy for non-invasive measurement and manipulation of live cells. Optics Express . ISSN 1094-4087 (In Press)

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Abstract

We present a new approach for combining holographic optical tweezers with confocal Raman spectroscopy. Multiple laser foci, generated using a liquid-crystal spatial light modulator, are individually used for both optical trapping and excitation of spontaneous Raman spectroscopy from trapped objects. Raman scattering from each laser focus is spatially filtered using reflective apertures on a digital micro-mirror device, which can be reconfigured with flexible patterns at video rate. We discuss operation of the instrument, and performance and viability considerations for biological measurements. We then demonstrate the capability of the instrument for fast, flexible, and interactive manipulation with molecular measurement of interacting live cell systems.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Physics and Astronomy
Depositing User: Eprints, Support
Date Deposited: 12 Sep 2018 12:51
Last Modified: 12 Sep 2018 12:51
URI: http://eprints.nottingham.ac.uk/id/eprint/54426

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