Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels

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Sander, Katrin N., Hay, Daniel P., Broughton Pipkin, Fiona, Khan, Raheela N. and Barrett, David A. (2018) Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels. Journal of Pharmaceutical and Biomedical Analysis, 160 . pp. 297-300. ISSN 1873-264X

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Official URL: https://www.sciencedirect.com/science/article/pii/S0731708518311373?via%3Dihub

Abstract

The analysis of lipids in tough or fibrous biological tissues can be challenging due to difficulties in obtaining a representative sample following homogenisation of the tissue. Furthermore, the choice of normalisation method can have a major effect on the quality of quantitative results. Therefore, a range of mechanical homogenisation techniques and normalisation strategies were evaluated for application to human placental vessels. The findings showed that rotor-stator homogenisation in a suitable solvent and wet weight normalisation were the best combination of procedures for quantitative analysis of lipids in placental blood vessels.

Item Type:	Article
Keywords:	Eicosanoids; Oxylipins
Schools/Departments:	University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number:	10.1016/j.jpba.2018.08.012
Depositing User:	Hatton, Mrs Kirsty
Date Deposited:	23 Aug 2018 10:34
Last Modified:	07 Aug 2019 04:30
URI:	https://eprints.nottingham.ac.uk/id/eprint/53439

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