Root gravitropism: quantification, challenges, and solutions

Muller, Lukas, Bennett, Malcolm J., French, Andy, Wells, Darren M. and Swarup, Ranjan (2018) Root gravitropism: quantification, challenges, and solutions. Methods in Molecular Biology, 1761 . pp. 103-112. ISSN 1940-6029

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Better understanding of root traits such as root angle and root gravitropism will be crucial for development of crops with improved resource use efficiency. This paper describes a high-throughput, automated image analysis method to trace Arabidopsis (Arabidopsis thaliana) seedling roots grown on agar plates. The method combines a ‘particle-filtering algorithm with a graph-based method’ to trace the centre line of a root and can be adopted for the analysis of several root parameters such as length, curvature, and stimulus from original root traces.

Item Type: Article
Keywords: Root gravitropism, RooTrace, Image analysis, Arabidopsis
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences > Division of Plant and Crop Sciences
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Depositing User: Eprints, Support
Date Deposited: 13 Jul 2018 09:59
Last Modified: 11 Mar 2019 04:30

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