Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support

Carucci, Cristina and Bruen, Larah and Gascón, Victoria and Paradisi, Francesca and Magner, Edmond (2018) Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support. Langmuir . ISSN 0743-7463

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Abstract

The use of an in-situ immobilization procedure for the immobilization of hyperhalophilic alcohol dehydrogenase in a metal organic framework material is described. The easy and rapid in situ immobilization process enables retention of activity over a broad range of pH and temperature together with a decrease in the halophilicity of the enzyme. The catalytic activity of the immobilized enzyme was studied in non-aqueous solvent mixtures with the highest retention of activity in aqueous solutions of methanol and acetonitrile. The approach demonstrates that this immobilization method can be extended to hyperhalophilic enzymes with enhancements in activity and stability.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/939176
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Chemistry
Identification Number: https://doi.org/10.1021/acs.langmuir.8b01037
Depositing User: Smith, Ruth
Date Deposited: 13 Jul 2018 10:39
Last Modified: 04 May 2020 19:41
URI: http://eprints.nottingham.ac.uk/id/eprint/52945

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