Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy

Penon, Oriol and Marín, María J. and Russell, David A. and Pérez-García, Lluïsa (2017) Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy. Journal of Colloid and Interface Science, 496 . pp. 100-110. ISSN 1095-7103

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Abstract

Photodynamic therapy (PDT) is a treatment of cancer by which tumour cells are destroyed using reactive oxygen species produced by photosensitizers following activation with visible or near infrared light. Successful PDT depends on the solubility and the targeting ability of the photosensitizers. In this work, the synthesis of a porphyrin-based water soluble nanoparticle conjugate containing a targeting agent that recognizes the erbB2 receptor overexpressed on the surface of particular cancer cells is reported. The nanoparticle conjugates were synthesized following two different protocols, viz. a biphasic and a monophasic method, with the aim to determine which method yielded the optimal nanosystem for potential PDT applications. The nanoparticles were characterized using UV–Vis absorption and fluorescence spectroscopies together with transmission electron microscopy and zeta potential measurements; and their ability to produce singlet oxygen following irradiation was investigated following the decay in absorption of a singlet oxygen probe. The nanoparticles synthesized using the monophasic method were shown to produce the highest amount of singlet oxygen and were further functionalized with anti-erbB2 antibody to target the erbB2 receptors expressed on the surface of SK-BR-3 human breast cancer cells. The water soluble, antibody-porphyrin nanoparticle conjugates were shown to elicit targeted PDT of the breast cancer cells.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/865994
Keywords: Photodynamic therapy ; Multifunctional nanoparticles ; Porphyrins ; Gold nanoparticles (AuNPs) ; Water soluble nanoconjugates ; Targeting antibodies ; Singlet oxygen production ; In vitro phototoxicity ; Human breast cancer cells
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: https://doi.org/10.1016/j.jcis.2017.02.006
Depositing User: Perez Garcia, Lluisa
Date Deposited: 12 Jul 2018 12:36
Last Modified: 04 May 2020 18:50
URI: http://eprints.nottingham.ac.uk/id/eprint/52917

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