Optical sectioning in multi-foci Raman hyperspectral imaging

Liao, Zhiyu and Sinjab, Faris and Elsheikha, Hany M. and Notingher, Ioan (2018) Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy . ISSN 1097-4555

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution.
Download (951kB) | Preview

Abstract

In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was employed for the generation of multi-foci laser beams, and a digital micromirror device (DMD) was used as a software-configurable reflective pinhole array. The patterns of the laser-foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance-to-size ratios were tested and compared. After optimisation of the laser foci pattern, we demonstrated the feasibility of multi-foci Raman hyperspectral microscopy for recording depth-resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth-discrimination and short acquisition times (20 minutes for single plane confocal image) was achieved.

Item Type: Article
Keywords: Confocal Raman imaging; optical sectioning; multi-beam; cross-talk; single cell imaging
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Physics and Astronomy
Identification Number: https://doi.org/10.1002/jrs.5450
Depositing User: Liao, Zhiyu
Date Deposited: 05 Jul 2018 08:34
Last Modified: 20 Jul 2018 14:00
URI: http://eprints.nottingham.ac.uk/id/eprint/52774

Actions (Archive Staff Only)

Edit View Edit View