Optical sectioning in multi-foci Raman hyperspectral imagingTools Liao, Zhiyu, Sinjab, Faris, Elsheikha, Hany M. and Notingher, Ioan (2018) Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy . ISSN 1097-4555 Full text not available from this repository.AbstractIn this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was employed for the generation of multi-foci laser beams, and a digital micromirror device (DMD) was used as a software-configurable reflective pinhole array. The patterns of the laser-foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance-to-size ratios were tested and compared. After optimisation of the laser foci pattern, we demonstrated the feasibility of multi-foci Raman hyperspectral microscopy for recording depth-resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth-discrimination and short acquisition times (20 minutes for single plane confocal image) was achieved.
Actions (Archive Staff Only)
|
Tools
Tools
