DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase

Killelea, Tom and Hawkins, Michelle and Howard, Jamieson and McGlynn, Peter and Bolt, Edward L. (2018) DNA replication roadblocks caused by Cascade Interference complexes are alleviated by RecG DNA repair helicase. RNA Biology . ISSN 1555-8584

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Abstract

Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/942648
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1080/15476286.2018.1496773
Related URLs:
URLURL Type
https://www.tandfonline.com/loi/krnb20UNSPECIFIED
Depositing User: Bolt, Ed
Date Deposited: 04 Jul 2018 08:01
Last Modified: 04 May 2020 19:42
URI: http://eprints.nottingham.ac.uk/id/eprint/52737

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