Epithelial IL-6 trans-signaling defines a new asthma phenotype with increased airway inflammation

Jevnikar, Zala and Östling, Jörgen and Ax, Elisabeth and Calvén, Jenny and Thörn, Kristofer and Israelsson, Elisabeth and Öberg, Lisa and Singhania, Akul and Lau, Laurie C.K. and Wilson, Susan J. and Ward, Jonathan A. and Chauhan, Anoop and Sousa, Ana R. and De Meulder, Bertrand and Loza, Matthew J. and Baribaud, Frédéric and Sterk, Peter J. and Chung, Kian Fan and Sun, Kai and Guo, Yike and Adcock, Ian M. and Payne, Debbie and Dahlen, Barbro and Chanez, Pascal and Shaw, Dominick E. and Krug, Norbert and Hohlfeld, Jens M. and Sandström, Thomas and Djukanovic, Ratko and James, Anna and Hinks, Timothy S.C. and Howarth, Peter H. and Vaarala, Outi and van Geest, Marleen and Olsson, Henric K. (2018) Epithelial IL-6 trans-signaling defines a new asthma phenotype with increased airway inflammation. Journal of Allergy and Clinical Immunology . ISSN 0091-6749

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Abstract

Background: Although several studies link high levels of IL-6 and soluble IL-6 receptor (sIL-6R) with asthma severity and decreased lung function, the role of IL-6 trans-signaling (IL-6TS) in asthma is unclear.

Objective: To explore the association between epithelial IL-6TS pathway activation and molecular and clinical phenotypes in asthma.

Methods: An IL-6TS gene signature, obtained from air-liquid interface (ALI) cultures of human bronchial epithelial cells stimulated with IL-6 and sIL-6R, was used to stratify lung epithelium transcriptomic data (U-BIOPRED cohorts) by hierarchical clustering. IL-6TS-specific protein markers were used to stratify sputum biomarker data (Wessex cohort). Molecular phenotyping was based on transcriptional profiling of epithelial brushings, pathway analysis and immunohistochemical analysis of bronchial biopsies.

Results: Activation of IL-6TS in ALI cultures reduced epithelial integrity and induced a specific gene signature enriched in genes associated with airway remodeling. The IL-6TS signature identified a subset of IL-6TS. High asthma patients with increased epithelial expression of IL-6TS inducible genes in absence of systemic inflammation. The IL-6TS High subset had an overrepresentation of frequent exacerbators, blood eosinophilia, and submucosal infiltration of T cells and macrophages. In bronchial brushings, TLR pathway genes were up-regulated while the expression of tight junction genes was reduced. Sputum sIL-6R and IL-6 levels correlated with sputum markers of remodeling and innate immune activation, in particular YKL-40, MMP3, MIP-1β, IL-8 and IL-1β.

Conclusions: Local lung epithelial IL-6TS activation in absence of type 2 airway inflammation defines a novel subset of asthmatics and may drive airway inflammation and epithelial dysfunction in these patients.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/937322
Keywords: Asthma, lung epithelium, transcriptomics, hierarchical clustering, IL-6 signaling, exacerbation frequency, eosinophils, airway inflammation, remodeling, epithelial integrity
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Respiratory Medicine
Identification Number: https://doi.org/10.1016/j.jaci.2018.05.026
Depositing User: Eprints, Support
Date Deposited: 14 Jun 2018 10:48
Last Modified: 04 May 2020 19:40
URI: http://eprints.nottingham.ac.uk/id/eprint/52416

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