A multimodal imaging approach enables in vivo assessment of antifungal treatment in a mouse model of invasive pulmonary aspergillosis

Poelmans, Jennifer and Himmelreich, Uwe and Vanherp, Liesbeth and Zhai, Luca and Hillen, Amy and Holvoet, Bryan and Belderbos, Sarah and Brock, Matthias and Maertens, Johan and Velde, Greetje Vande and Lagrou, Katrien (2018) A multimodal imaging approach enables in vivo assessment of antifungal treatment in a mouse model of invasive pulmonary aspergillosis. Antimicrobial Agents and Chemotherapy . AAC.00240-18. ISSN 0066-4804

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Abstract

Aspergillus fumigatus causes life-threatening lung infections in immunocompromised patients. Mouse models are extensively used in research to assess the in vivo efficacy of antifungals. In recent years, there has been an increasing interest in the use of non-invasive imaging techniques to evaluate experimental infections. However, single imaging modalities have limitations concerning the type of information they can provide. In this study, magnetic resonance imaging and bioluminescence imaging were combined to obtain longitudinal information on the extent of developing lesions and fungal load in a leucopenic mouse model of IPA. This multimodal imaging approach was used to assess changes occurring within lungs of infected mice receiving voriconazole treatment starting at different time points after infection. Results showed that IPA development depends on the inoculum size used to infect animals and that disease can be successfully prevented or treated by initiating intervention during early stages of infection. Furthermore, we demonstrated that reduction of the fungal load is not necessarily associated with the disappearance of lesions on anatomical lung images, especially when antifungal treatment coincides with immune recovery. In conclusion, multimodal imaging allows to investigate different aspects of disease progression or recovery by providing complementary information on dynamic processes, which are highly useful for assessing the efficacy of (novel) therapeutic compounds in a time- and labor-efficient manner.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/932266
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Life Sciences
Identification Number: https://doi.org/10.1128/AAC.00240-18
Depositing User: Eprints, Support
Date Deposited: 13 Jun 2018 13:44
Last Modified: 04 May 2020 19:36
URI: http://eprints.nottingham.ac.uk/id/eprint/52384

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