Rapid one-step biotinylation of biological and non-biological surfaces

Henry, Stephen and Williams, Eleanor and Barr, Katie and Korchagina, Elena and Tuzikov, Alexandr and Ilyushina, Natalia and Abayzeed, Sidahmed A. and Webb, Kevin F. and Bovin, Nicolai (2018) Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8 (1). 2845/1-2845/6. ISSN 2045-2322

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Abstract

We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/911223
Schools/Departments: University of Nottingham, UK > Faculty of Engineering > Department of Electrical and Electronic Engineering
Identification Number: https://doi.org/10.1038/s41598-018-21186-3
Depositing User: Eprints, Support
Date Deposited: 06 Jun 2018 09:31
Last Modified: 04 May 2020 19:31
URI: http://eprints.nottingham.ac.uk/id/eprint/52262

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