Rapid one-step biotinylation of biological and non-biological surfacesTools Henry, Stephen, Williams, Eleanor, Barr, Katie, Korchagina, Elena, Tuzikov, Alexandr, Ilyushina, Natalia, Abayzeed, Sidahmed A., Webb, Kevin F. and Bovin, Nicolai (2018) Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8 (1). 2845/1-2845/6. ISSN 2045-2322 Full text not available from this repository.
Official URL: https://doi.org/10.1038/s41598-018-21186-3
AbstractWe describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.
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