High-throughput miniaturized screening of nanoparticle formation via inkjet printing

Styliari, Ioanna D. and Conte, Claudia and Pearce, Amanda K. and Hüsler, Amanda and Cavanagh, Robert J. and Limo, Marion J. and Gordhan, Dipak and Nieto-Orellana, Alejandro and Suksiriworapong, Jiraphong and Couturaud, Benoit and Williams, Phil and Hook, Andrew L. and Alexander, Morgan R. and Garnett, Martin C. and Alexander, Cameron and Burley, Jonathan C. and Taresco, Vincenzo (2018) High-throughput miniaturized screening of nanoparticle formation via inkjet printing. Macromolecular Materials and Engineering, 303 (8). ISSN 1439-2054

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Abstract

The self‐assembly of specific polymers into well‐defined nanoparticles (NPs) is of great interest to the pharmaceutical industry as the resultant materials can act as drug delivery vehicles. In this work, a high‐throughput method to screen the ability of polymers to self‐assemble into NPs using a picoliter inkjet printer is presented. By dispensing polymer solutions in dimethyl sulfoxide (DMSO) from the printer into the wells of a 96‐well plate, containing water as an antisolvent, 50 suspensions are screened for nanoparticle formation rapidly using only nanoliters to microliters. A variety of polymer classes are used and in situ characterization of the submicroliter nanosuspensions shows that the particle size distributions match those of nanoparticles made from bulk suspensions. Dispensing organic polymer solutions into well plates via the printer is thus shown to be a reproducible and fast method for screening nanoparticle formation which uses two to three orders of magnitude less material than conventional techniques. Finally, a pilot study for a high‐throughput pipeline of nanoparticle production, physical property characterization, and cytocompatibility demonstrates the feasibility of the printing approach for screening of nanodrug delivery formulations. Nanoparticles are produced in the well plates, characterized for size and evaluated for effects on metabolic activity of lung cancer cells.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/934364
Additional Information: This is the accepted version of the following article: I. D. Styliari, C. Conte, A. K. Pearce, A. Hüsler, R. J. Cavanagh, M. J. Limo, D. Gordhan, A. Nieto‐Orellana, J. Suksiriworapong, B. Couturaud, P. Williams, A. L. Hook, M. R. Alexander, M. C. Garnett, C. Alexander, J. C. Burley, V. Taresco, Macromol. Mater. Eng. 2018, 303, 1800146. https://doi.org/10.1002/mame.201800146, which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/mame.201800146. This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy.
Keywords: High-throughput miniaturized screening; Inkjet printers; Nanoparticles; Self-assembling
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: https://doi.org/10.1002/mame.201800146
Depositing User: Eprints, Support
Date Deposited: 05 Jun 2018 10:31
Last Modified: 04 May 2020 19:37
URI: http://eprints.nottingham.ac.uk/id/eprint/52245

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