Self-sustaining closed-loop multienzyme mediated conversion of amines into alcohols in continuous reactions

Contente, Martina L. and Paradisi, Francesca (2018) Self-sustaining closed-loop multienzyme mediated conversion of amines into alcohols in continuous reactions. Nature Catalysis . ISSN 2520-1158

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Abstract

The synthesis of alcohols from amines starting material is an excellent, yet challenging, strategy for the preparation of pharmaceuticals and polymers. Here, we developed a versatile, self-sustaining closed-loop multienzymatic platform for the biocatalytic synthesis of a large range of non-commercially available products in continuous flow with excellent yields (80>99%), reaction times and optical purity of secondary alcohols (>99 e.e.). This process was also extended to the conversion of biogenic amines into high value alcohols, such as the powerful anti-oxidant hydroxytyrosol, and the synthesis of enantiopure 2-arylpropanols via dynamic kinetic resolution of commercially affordable racemic amines. The system exploits the in situ immobilisation of transaminases and redox enzymes which were combined to cater for a fully automated, ultra-efficient synthetic platform with cofactor recycling, in-line recovery of benign by-products and recirculation of the aqueous media containing the recycled cofactors in catalytic amount, which increases the efficiency of the system by over 20-fold.

Item Type: Article
RIS ID: https://nottingham-repository.worktribe.com/output/936457
Keywords: Biocatalysis; Enzymes; Flow chemistry; Synthetic chemistry; methodology; Sustainability
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Chemistry
Identification Number: https://doi.org/10.1038/s41929-018-0082-9
Depositing User: Smith, Ruth
Date Deposited: 06 Jun 2018 13:54
Last Modified: 04 May 2020 19:39
URI: http://eprints.nottingham.ac.uk/id/eprint/51950

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