CD10 inhibits cell motility but expression is associated with advanced stage disease in colorectal cancer

Raposo, T.P. and Comes, Mireia Sueca and Idowu, Adeyemi and Agit, Bora and Hassall, James and Fadhil, Wakkas and Nica, Robert and Ecker, Rupert and Yao, Takashi and Ilyas, Mohammad (2018) CD10 inhibits cell motility but expression is associated with advanced stage disease in colorectal cancer. Experimental and Molecular Pathology, 104 (3). pp. 190-198. ISSN 1096-0945

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Abstract

Introduction

CD10 is a cell membrane-bound endopeptidase which is expressed in normal small bowel but not in normal colon. It is aberrantly expressed in a small proportion of colorectal cancers (CRC) and this has been associated with liver metastasis and poor prognosis. We sought to investigate the mechanism of CD10 activity and its association with clinicopathological features.

Material and methods

CD10 was stably knocked down by lentiviral shRNA transduction in the CRC cell lines SW480 and SW620 which are derived from a primary tumour and its corresponding metastasis respectively. Expression of epithelial – mesenchymal transition (EMT) markers was tested as well as the effect of knockdown on cell viability, migration and invasion assays. In addition, immunohistochemical expression of CD10 in primary colorectal tumours (N = 84) in a tissue microarray was digitally quantified and analysed for associations with clinicopathological variables.

Results

Knockdown of CD10 did not alter cell viability in SW480, but migration and invasion levels increased (P < 0.001 for each) and this was associated with a cadherin switch. In SW620, CD10 knockdown caused a reduction in cell viability after 72 h (P = 0.0018) but it had no effect on cell migration and invasion. Expression of epithelial CD10 in primary tumours was associated with presence of lymph node invasion (P = 0.001) and advanced Duke's stage (P = 0.001).

Conclusions

Our results suggest that the function of CD10 may change during tumour evolution. It may inhibit cell motility in early-stage disease whilst promoting cell viability in late-stage disease. It has a complex role and further studies are needed to elucidate the suitability of CD10 as a prognostic marker or therapeutic target.

Item Type: Article
Keywords: CD10; NEP; CALLA; Colorectal cancer; Epithelial-mesenchymal transition; Metastasis
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Cancer and Stem Cells
Identification Number: https://doi.org/10.1016/j.yexmp.2018.04.002
Depositing User: Eprints, Support
Date Deposited: 25 Apr 2018 08:07
Last Modified: 11 Apr 2019 04:30
URI: http://eprints.nottingham.ac.uk/id/eprint/51365

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