Opsonic phagocytosis in chronic obstructive pulmonary disease is enhanced by Nrf2 agonists

Bewley, Martin A. and Budd, Richard C. and Ryan, Eilise and Cole, Joby and Collini, Paul and Marshall, Jennifer and Kolsum, Umme and Beech, Gussie and Emes, Richard D. and Tcherniaeva, Irina and Berbers, Gguy A.M. and Walmsley, Sarah R. and Donaldson, Gavin and Wedzicha, Jadwiga A. and Kilty, Iain and Rumsey, William and Sanchez, Yolanda and Brightling, Christopher E. and Donnelly, Louise E. and Barnes, Ppeter J. and Singh, Dave and Whyte, Moira K.B. and Dockrell, David H. (2018) Opsonic phagocytosis in chronic obstructive pulmonary disease is enhanced by Nrf2 agonists. American Journal of Respiratory and Critical Care Medicine . ISSN 1073-449X

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Download (3MB) | Preview

Abstract

Rationale: Previous studies have identified defects in bacterial phagocytosis by alveolar macrophages (AM) in patients with chronic obstructive pulmonary disease (COPD) but the mechanisms and clinical consequences remain incompletely defined.

Objectives: To examine the effect of COPD on AM phagocytic responses and identify the mechanisms, clinical consequences and potential for therapeutic manipulation of these defects.

Methods: We isolated alveolar macrophages (AM) and monocyte-derived macrophages (MDM) from a cohort of COPD patients and controls within the MRC COPD-MAP consortium and measured phagocytosis of bacteria in relation to opsonic conditions and clinical features.

Measurements and Main Results: COPD AM and MDM have impaired phagocytosis of S. pneumoniae. COPD AM have a selective defect in uptake of opsonized bacteria, despite the presence of anti-pneumococcal antibodies in bronchoalveolar lavage, not observed in MDM or healthy donor’s AM. AM defects in phagocytosis in COPD are significantly associated with exacerbation frequency, isolation of pathogenic bacteria and health related quality of life scores. Bacterial binding and initial intracellular killing of opsonized bacteria in COPD AM was not reduced. COPD AM have reduced transcriptional responses to opsonized bacteria, including cellular stress responses that include transcriptional modules involving antioxidant defenses and Nrf2-regualted genes. Agonists of the cytoprotective transcription factor Nrf2 (sulforaphane and Compound) reverse defects in phagocytosis of S. pneumoniae and non-type able Haemophilus influenzae by COPD.

Conclusions: Patients with COPD have clinically relevant defects in opsonic phagocytosis by AM, associated with impaired transcriptional responses to cellular stress, which are reversed by therapeutic targeting with Nrf2 agonists.

Item Type: Article
Keywords: COPD ; Macrophage ; Phagocytosis ; Anti-oxidant ; Nrf2
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Veterinary Medicine and Science
Identification Number: https://doi.org/10.1164/rccm.201705-0903OC
Depositing User: Emes, Richard
Date Deposited: 20 Apr 2018 10:11
Last Modified: 16 Mar 2019 04:30
URI: http://eprints.nottingham.ac.uk/id/eprint/51277

Actions (Archive Staff Only)

Edit View Edit View