Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals

McIvor, Emma and Coombes, Stephen and Thul, Ruediger (2018) Three-dimensional spatio-temporal modelling of store operated Ca2+ entry: insights into ER refilling and the spatial signature of Ca2+ signals. Cell Calcium, 73 . pp. 11-24. ISSN 1532-1991

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Abstract

The spatial organisation of Orai channels and SERCA pumps within ER-PM junctions is important for enhancing the versatility and specificity of subcellular Ca2+ signals generated during store operated Ca2+ entry (SOCE). In this paper we present a novel three dimensional spatio-temporal model describing Ca2+ dynamics in the ER-PM junction and sub-PM ER during SOCE. We investigate the role of Orai channel and SERCA pump location to provide insights into how these components shape the Ca2+ signals generated and affect ER refilling. We find that the organisation of Orai channels within the ER-PM junction controls the amplitude and shape of the Ca2+ profile but does not enhance ER refilling. The model shows that ER refilling is only weakly affected by the location of SERCA2b pumps within the ER-PM junction and that the placement of SERCA2a pumps within the ER-PM junction has much greater control over ER refilling.

Item Type: Article
Keywords: Calcium signalling; ER-PM junctions; Mathematical modelling; Spatial Ca2+ signature; Orai channels; ER refilling
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Mathematical Sciences
Identification Number: 10.1016/j.ceca.2018.03.006
Depositing User: Thul, Ruediger
Date Deposited: 16 Apr 2018 12:57
Last Modified: 16 Apr 2018 13:36
URI: http://eprints.nottingham.ac.uk/id/eprint/51146

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