Characterisation of insulin analogues therapeutically available to patients

Adams, Gary G. and Meal, Andrew and Morgan, Paul S. and Alzahrani, Qushmua E. and Zobel, Hanne and Lithgo, Ryan and Kok, M. Samil and Besong, David T.M. and Jiwani, Shahwar I. and Ballance, Simon and Harding, Stephen E. and Gillis, Richard B. and Chayen, Naomi (2018) Characterisation of insulin analogues therapeutically available to patients. PLoS ONE, 13 (3). e0195010/1-e0195010/17. ISSN 1932-6203

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution.
Download (1MB) | Preview

Abstract

The structure and function of clinical dosage insulin and its analogues were assessed. This included ‘native insulins’ (human recombinant, bovine, porcine), ‘fast-acting analogues’ (aspart, glulisine, lispro) and ‘slow-acting analogues’ (glargine, detemir, degludec). Analytical ultracentrifugation, both sedimentation velocity and equilibrium experiments, were employed to yield distributions of both molar mass and sedimentation coefficient of all nine insulins. Size exclusion chromatography, coupled to multi-angle light scattering, was also used to explore the function of these analogues. On ultracentrifugation analysis, the insulins under investigation were found to be in numerous conformational states, however the majority of insulins were present in a primarily hexameric conformation. This was true for all native insulins and two fast-acting analogues. However, glargine was present as a dimer, detemir was a multi-hexameric system, degludec was a dodecamer (di-hexamer) and glulisine was present as a dimer-hexamer-dihexamer system. However, size-exclusion chromatography showed that the two hexameric fast-acting analogues (aspart and lispro) dissociated into monomers and dimers due to the lack of zinc in the mobile phase. This comprehensive study is the first time all nine insulins have been characterised in this way, the first time that insulin detemir have been studied using analytical ultracentrifugation and the first time that insulins aspart and glulisine have been studied using sedimentation equilibrium. The structure and function of these clinically administered insulins is of critical importance and this research adds novel data to an otherwise complex functional physiological protein.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences
University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: https://doi.org/10.1371/journal.pone.0195010
Depositing User: Eprints, Support
Date Deposited: 09 Apr 2018 10:05
Last Modified: 02 Jul 2018 09:19
URI: http://eprints.nottingham.ac.uk/id/eprint/51022

Actions (Archive Staff Only)

Edit View Edit View