Synthesis and in vitro phototoxicity of multifunctional Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin-coated gold nanoparticles assembled via axial coordination with imidazole ligands

Alea-Reyes, Maria E. and Penon, Oriol and García Calavia, Paula and Marín, María J. and Russell, David A. and Pérez-García, Lluïsa (2018) Synthesis and in vitro phototoxicity of multifunctional Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin-coated gold nanoparticles assembled via axial coordination with imidazole ligands. Journal of Colloid and Interface Science, 521 . pp. 81-90. ISSN 1095-7103

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Abstract

Hypothesis

Metalloporphyrins are extensively investigated for their ability to form reactive oxygen species and as potent photosensitisers for use in photodynamic therapy. However, their hydrophobicity generally causes solubility issues concerning in vivo delivery due to lack of distribution and low clearance from the body. Immobilising porphyrins on carriers, such as gold nanoparticles (GNP), can overcome some of these drawbacks. The mode of assembling the porphyrins to the carrier influences the properties of the resulting drug delivery systems.

Experiments

We describe the synthesis and characterisation of new porphyrin decorated water soluble GNP and we explore Zn-imidazole axial coordination as the mode of linking the porphyrin to the metallic core of the nanoparticles. Quantification of singlet oxygen production, toxicity in dark, cellular uptake by SK-BR-3 cells and phototoxicity have been assessed.

Findings

Axial coordination limits the number of porphyrins on the gold surface, reduces the formation of aggregates, and diminishes metal exchange in the porphyrin, all of which contribute to enhance the efficiency of singlet oxygen generation from the immobilised porphyrin. In vitro experiments on SK-BR-3 cells reveal a fast uptake followed by more than 80% cell death after irradiation with low doses of light.

Item Type: Article
Keywords: Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin; Gold nanoparticles; Imidazole ligands Zn(II)-imidazole axial coordination; Singlet oxygen production; SK-BR-3 cells; Cellular uptake; Dark cytotoxicity; Phototoxiciy; Photodynamic therapy
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Pharmacy
Identification Number: 10.1016/j.jcis.2018.03.014
Depositing User: Perez Garcia, Lluisa
Date Deposited: 04 Apr 2018 10:55
Last Modified: 04 Apr 2018 10:55
URI: http://eprints.nottingham.ac.uk/id/eprint/50850

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