Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation

Wakefield, Jennifer M.K. and Gillis, Richard B. and Adams, Gary G. and McQueen, Caitlin and Harding, Stephen E. (2018) Controlled depolymerisation, as assessed by analytical ultracentrifugation, of low molecular weight chitosan for potential use in archaeological conservation. European Biophysics Journal . ISSN 1432-1017

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Abstract

The heterogeneity and molecular weight of a chitosan of low molecular weight (molar mass) and low degree of acetylation (0.1), for potential use as a consolidant for decayed archaeological wood, has been examined by sedimentation velocity and sedimentation equilibriumin the analytical ultracentrifuge before and after depolymerisation. Sedimentation velocity before polymerisation revealed a uniform distribution of sedimentation coefficient with little concentration dependence. SEDFIT-MSTAR analysis revealed a weight average molecular weight Mw of (14.2 + 1.2) kDa, and polydispersity index of ~ 1.2. Further analysis using MULTISIG revealed a distribution of material between 2-20 kDa and consistent with the weight average Mw. Controlled depolymerisation using hydrogen peroxide and UV in an acetic acid medium reduced this to (4.9 + 0.7) kDa, with a similar polydispersity. The depolymerised material appears to be within the range that has been predicted to fully penetrate into archaeological wood. The consequences for this and the use of the analytical ultracentrifuge in wood conservation strategies is considered.

Item Type: Article
Keywords: Analytical ultracentrifuge, SEDFIT-MSTAR, hydrogen peroxide-ultraviolet depolymerisation, Oseberg artefacts
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Health Sciences
University of Nottingham, UK > Faculty of Science > School of Biosciences
University of Nottingham, UK > Faculty of Science > School of Chemistry
Identification Number: https://doi.org/10.1007/s00249-018-1290-6
Depositing User: Eprints, Support
Date Deposited: 15 Mar 2018 11:12
Last Modified: 02 Jul 2018 09:18
URI: http://eprints.nottingham.ac.uk/id/eprint/50463

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