Low temperature exposure induces browning of bone marrow stem cell derived adipocytes in vitro

Velickovic, Ksenija and Lugo-Leija, Hilda Anaid and Bloor, Ian and Law, James and Sacks, Harold and Symonds, Michael E. and Sottile, Virginie (2018) Low temperature exposure induces browning of bone marrow stem cell derived adipocytes in vitro. Scientific Reports, 8 . 4974/1-4674/12. ISSN 2045-2322 (In Press)

[img]
Preview
PDF - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader
Available under Licence Creative Commons Attribution.
Download (6MB) | Preview

Abstract

Brown and beige adipocytes are characterised as expressing the unique mitochondrial uncoupling protein (UCP)1 for which the primary stimulus in vivo is cold exposure. The extent to which cold-induced UCP1 activation can also be achieved in vitro, and therefore perform a comparable cellular function, is unknown. We report an in vitro model to induce adipocyte browning using bone marrow (BM) derived mesenchymal stem cells (MSC), which relies on differentiation at 32°C instead of 37°C. The low temperature promoted browning in adipogenic cultures, with increased adipocyte differentiation and upregulation of adipogenic and thermogenic factors, especially UCP1. Cells exhibited enhanced uncoupled respiration and metabolic adaptation. Cold-exposed differentiated cells showed a marked translocation of leptin to adipocyte nuclei, suggesting a previously unknown role for leptin in the browning process. These results indicate that BM-MSC can be driven to forming beige-like adipocytes in vitro by exposure to a reduced temperature. This in vitro model will provide a powerful tool to elucidate the precise role of leptin and related hormones in hitherto functions in the browning process.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Child Health, Obstetrics and Gynaecology
Identification Number: 10.1038/s41598-018-23267-9
Depositing User: Eprints, Support
Date Deposited: 22 Mar 2018 09:08
Last Modified: 22 Mar 2018 12:19
URI: http://eprints.nottingham.ac.uk/id/eprint/50341

Actions (Archive Staff Only)

Edit View Edit View