FISSA: A neuropil decontamination toolbox for calcium imaging signals

Keemink, Sander W. and Lowe, Scott C. and Pakan, Janelle M.P. and Dylda, Evelyn and van Rossum, Mark C.W. and Rochefort, Nathalie L. (2018) FISSA: A neuropil decontamination toolbox for calcium imaging signals. Scientific Reports, 8 . p. 3493. ISSN 2045-2322

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Abstract

In vivo calcium imaging has become a method of choice to image neuronal population activity throughout the nervous system. These experiments generate large sequences of images. Their analysis is computationally intensive and typically involves motion correction, image segmentation into regions of interest (ROIs), and extraction of fluorescence traces of each ROI. Out of focus fluorescence from surrounding neuropil and other cells can strongly contaminate the signal assigned to a given ROI. In this study, we introduce the FISSA toolbox (Fast Image Signal Separation Analysis) for neuropil decontamination. Given pre-defined ROIs, the FISSA toolbox automatically extracts the surrounding local neuropil and performs blind-source separation with non-negative matrix factorization. Using both simulated and in vivo data, we show that this toolbox performs similarly or better than existing published methods. FISSA requires only little RAM, allowing for fast processing of large datasets even on a standard laptop. The FISSA toolbox is available in Python, with an option for MATLAB format outputs, and can easily be integrated into existing workflows. It is available from Github and the standard Python repositories.

Item Type: Article
Keywords: calcium imaging, two-photon, source separation, signal extraction, neuropil contamination, NMF, GCamP6
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Mathematical Sciences
University of Nottingham, UK > Faculty of Science > School of Psychology
Identification Number: https://doi.org/10.1038/s41598-018-21640-2
Depositing User: Van Rossum, Mark
Date Deposited: 22 Feb 2018 13:07
Last Modified: 12 Jun 2018 03:58
URI: http://eprints.nottingham.ac.uk/id/eprint/49936

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