Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin

Rashidi, Hassan and Strohbuecker, Stephanie and Jackson, Leigh and Kalra, Spandan and Blake, Alexander J. and France, Louise and Tufarelli, Cristina and Sottile, Virginie (2011) Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin. Cells Tissues Organs, 195 (6). pp. 484-94. ISSN 1422-6421

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Abstract

Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling.

Item Type: Article
Schools/Departments: University of Nottingham, UK > Faculty of Medicine and Health Sciences > School of Medicine > Division of Cancer and Stem Cells
Identification Number: https://doi.org/10.1159/000329861
Depositing User: Sottile, Virginie
Date Deposited: 22 Feb 2018 15:31
Last Modified: 22 Feb 2018 15:31
URI: http://eprints.nottingham.ac.uk/id/eprint/49919

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