Comparative genomics of bacteriophage of the genus Seuratvirus

Sazinas, Pavelas and Redgwell, Tamsin and Rihtman, Branko and Grigonyte, Aurelija and Michniewski, Slawomir and Scanlan, David J. and Hobman, Jon L. and Millard, Andrew (2017) Comparative genomics of bacteriophage of the genus Seuratvirus. Genome Biology and Evolution, 10 (1). pp. 72-76. ISSN 1759-6653

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Abstract

Despite being more abundant and having smaller genomes than their bacterial host, relatively few bacteriophages have had their genomes sequenced. Here, we isolated 14 bacteriophages from cattle slurry and performed de novo genome sequencing, assembly, and annotation. The commonly used marker genes polB and terL showed these bacteriophages to be closely related to members of the genus Seuratvirus. We performed a core-gene analysis using the 14 new and four closely related genomes. A total of 58 core genes were identified, the majority of which has no known function. These genes were used to construct a core-gene phylogeny, the results of which confirmed the new isolates to be part of the genus Seuratvirus and expanded the number of species within this genus to four. All bacteriophages within the genus contained the genes queCDE encoding enzymes involved in queuosine biosynthesis. We suggest these genes are carried as a mechanism to modify DNA in order to protect these bacteriophages against host endonucleases.

Item Type: Article
Keywords: bacteriophage, genomics, evolution
Schools/Departments: University of Nottingham, UK > Faculty of Science > School of Biosciences
Identification Number: 10.1093/gbe/evx275
Depositing User: Eprints, Support
Date Deposited: 09 Jan 2018 09:42
Last Modified: 09 Jan 2018 21:07
URI: http://eprints.nottingham.ac.uk/id/eprint/48991

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